FIG. 4.

(Top) Binding of labeled M. conjunctivae to LSM 192 cells without competing peptides (untreated), in the presence of peptide VDARGDVYK (RGD), in the presence of peptide VDARADVYK (RAD), and in the presence of fibronectin-derived peptide GRGDSP (RGD-fibro) at two different concentrations. Binding of M. conjunctivae to LSM 192 cells in the absence of peptides is referred to as 100%. The data shown are mean values from 5 independent measurements. Standard errors calculated are indicated by error bars. Statistically significant differences between the groups and the untreated sample are marked with asterisks, and P values are given in the text. Standard errors are indicated by error bars. (Bottom) A comparison of the RGD-flanking amino acids between LppT and fibronectin is shown (the sequences of the peptides used in this experiment are in boldface).