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. 2010 May 10;78(7):3064–3072. doi: 10.1128/IAI.00123-10

FIG. 2.

FIG. 2.

Functional analysis of VirR boxes. (A) Gel mobility shift analysis of the VirR boxes upstream of netB. The DIG-labeled DNA targets containing the VirR boxes upstream of pfoA or netB were incubated in the absence (−) or presence (+) of 1 μg of purified VirR. The pJIR2373 vector control is denoted by V. The free DNA (F), CI, and CII bands are labeled and indicated by the arrows. (B) Perfringolysin O activity of reporter constructs. Perfringolysin O (PFO) titers of the JIR325 wild-type strain (WT), JIR4228 derivatives carrying reporter constructs containing the VirR boxes located upstream of the pfoA gene (pfoA VB) and the netB gene (netB VB), and JIR4228 derivatives with the shuttle vector (pJIR751) and the reporter gene vector (pJIR2373) are shown. The error bars represent the standard error of the mean; n = 3.