FIG. 2.

Comparison of murine TLR4 activation by the lpxL mutants and the wild-type strain. HEK293 cells transfected with mouse TLR4, MD-2, and CD14 were stimulated with different concentrations of heat-inactivated whole H44/76 bacteria, lpxL1 bacteria, lpxL2 bacteria, lpxA bacteria, or purified LPS from these strains. After overnight incubation, the IL-8 concentration in the supernatant was measured by ELISA. Results of one representative experiment of three independent experiments are shown. Data are expressed as means of triplicates, and error bars indicate SEM.