FIG. 4.

Soluble components of B. burgdorferi spent medium govern IFN-responsive gene transcription by BMDMs. Cultured C3H/HeJ BMDMs were stimulated for 6 h with 5 μg/ml recombinant lipidated OspA, 7.4 × 10⁶/ml live B. burgdorferi organisms (Bb), 5 μg/ml sonicated B. burgdorferi, 10 ng/ml LPS, 50 to 150 μl of B. burgdorferi culture supernatant, or 50 to 150 μl of clean BSKII medium. Data are depicted as the means ± SEM and are representative of two independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA with the Tukey-Kramer multiple comparisons posttest. Differences in Ifit1(A) and Ifit2 (B) transcript levels between stimulated and unstimulated samples: *, P < 0.05; **, P < 0.01: ***, P < 0.001.