TABLE 1.
IFN-responsive gene induction by B. burgdorferi-stimulated BMDMs is STAT1 dependent
| Transcript | Levela (mean ± SEM) with BMDM genotype and stimulant: |
|||
|---|---|---|---|---|
| C3H/HeNb |
C3H STAT1−/−c |
|||
| Medium | Live B. burgdorferi | Medium | Live B. burgdorferi | |
| Cxcl9 | 0.2 ± 0.1 | 0.6 ± 0.1** | 0.0 ± 0.0 | 0.0 ± 0.0** |
| Cxcl10 | 2.6 ± 1.5 | 80.9 ± 10.8** | 0.3 ± 0.2 | 0.3 ± 0.1** |
| Gpb2 | 15.0 ± 4.6 | 110.6 ± 9.8** | 0.8 ± 0.2 | 1.6 ± 0.4** |
| Ifit1 | 13.9 ± 6.1 | 175.3 ± 35.6** | 0.1 ± 0.0 | 0.4 ± 0.1** |
a
Number of copies normalized to 1,000 copies of the mouse β-actin housekeeping gene. Data are pooled from three independent experiments (n = 6). Statistical significance was assessed by the Mann-Whitney U test.
b
**, P < 0.01 for live B. burgdorferi versus medium.
c
**, P < 0.01 for STAT1−/−/live B. burgdorferi versus C3H/HeN/live B. burgdorferi.