TABLE 2.
Nuclease treatment does not alter the IFN-stimulatory activity of B. burgdorferi culture supernatants
| Stimulant | Transcript level (mean ± SEM)a |
|
|---|---|---|
| Cxcl10 | Tyki | |
| Medium | 0.4 ± 0.0 | 3.0 ± 0.1 |
| B. burgdorferi RNAb | 4.2 ± 0.1*** | 17.3 ± 1.5* |
| B. burgdorferi RNA + RNasec | 1.0 ± 0.3** | 4.1 ± 0.2* |
| BSKII | 0.5 ± 0.1 | 2.6 ± 0.1 |
| B. burgdorferi culture supernatantd | 3.4 ± 0.1** | 9.0 ± 0.5** |
| B. burgdorferi supernatant + DNase | 5.2 ± 0.7 | 10.6 ± 2.1 |
| B. burgdorferi supernatant + RNase | 3.0 ± 0.2 | 7.4 ± 1.5 |
a
RT-PCR data are shown as the number of copies of the gene of interest per 1,000 copies of the β-actin gene and are representative of two independent experiments (n = 3). Statistical significance was assessed via a two-tailed, unpaired Student t test with the Welch correction.
b
For Cxcl10: ***, P < 0.001 versus medium. For Tyki: *, P < 0.05 versus medium.
c
For Cxcl10: **, P < 0.01 versus B. burgdorferi RNA. For Tyki: *, P < 0.05 versus B. burgdorferi RNA.
d
For Cxcl10: **, P < 0.01 versus BSKII. For Tyki: **, P < 0.01 versus BSKII.