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. 2010 May 10;78(7):3027–3035. doi: 10.1128/IAI.00264-10

FIG. 3.

FIG. 3.

Identification of immunoreactive proteins. (a) Supernatant proteins from ETEC strain H10407 separated by liquid-phase isoelectric focusing (pH gradient 3 to 10) followed by SDS-PAGE. (b) Immunoblot of panel a. (c) Gel image of outer membrane preparation from strain jf1412 (fliC mutant), separated in 2 dimensions. (d) Immunoblot of panel c. (Blots were developed with pooled sera from mice repeatedly infected with H10407.) The white numbers in black boxes on each panel correspond to selected individual immunoreactive protein bands chosen for identification. The protein band numbers (shown first) and proteins identified were as follows: 1, flagellin (E. coli, serotype, H11); 2, EatA autotransporter; 3, antigen 43 autotransporter protein; 4, DnaK (Hsp70) chaperone; 5, EtpA two-partner secretion exoprotein; 6, DegP membrane-associated serine protease; 7, OmpA; 8, CfaA periplasmic chaperone; 9, fimbrial usher; 10, outer membrane iron transport receptor.