TABLE 1.
Bacterial strains and plasmids used in these studies
| Bacterial strain or plasmid | Relevant genotype or descriptiona | Reference or source |
|---|---|---|
| Bacterial strains | ||
| H10407 | ETEC (Bangladesh) serotype O78:H11, LT+ ST+, CFA/I | 13 |
| ETP98116 | ETEC (ICDDR,B), ST+, CFA/I | This study |
| ETP98118 | ETEC (ICDDR,B), ST+, CFA/I | This study |
| ETP98087 | ETEC (ICDDR,B), LT+ ST+, CS2 CS3 | This study |
| jf1412 | H10407 fliC::Kmr mutant; nonmotile | 49 |
| E. coli Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araD139 Δ(ara leu)7697galUgalKrpsL (Strr) endA1nupG | Invitrogen |
| Plasmids | ||
| pJL017 | etpBA cloned into pBAD/Myc-His A, with etpA in frame with myc and six-His coding regions | 47 |
| pJL030 | etpC gene cloned into pACYC184; Cmr | 49 |
a
Abbreviations: ICDDR,B, International Centre for Diarrhoeal Disease Research, Bangladesh; LT+, heat-labile toxin positive; ST+, heat-stable toxin positive; CFA/I, colonization factor antigen I; CS2, coli surface antigen 2; Kmr, kanamycin resistance; Strr, streptomycin resistance; Cmr, chloramphenicol resistance.