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Invasiveness of S. enterica strains in Caco-2 cells. In all cases, a final concentration of 106 CFU ml−1 of either the wild type (wt) or the recAo6869 (recAo) mutant derivatives was inoculated into confluent monolayers of Caco-2 cells. After 1 h of incubation and a gentamicin treatment, Caco-2 cells were lysed; the number of invasive cells is shown. In all cases, the invasion assay was carried out in triplicate.
