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. 2010 Apr 26;78(7):3090–3096. doi: 10.1128/IAI.00034-10

FIG. 2.

FIG. 2.

NlpI deletion in E. coli K1 strain RS218 did not affect the expression of type 1 fimbriae, flagella, and OmpA. (A) The invertible element orientations assay revealed that the percentages of the cells with the fim promoter in the phase-ON orientation in the wild-type RS218 and the nlpI mutant were similar. The 198- and 160-bp DNA fragments represented the SnaBI digested phase-ON and phase-OFF products (see Materials and Methods). (B) Western blot analysis of FliC levels in the wild-type strain and the RS218 nlpI mutant, using anti-flagellin H7 antibodies. The expression of FliC was similar between the two strains. (C) The swimming motilities of the wild-type strain and the RS218 nlpI mutant on the 0.3% agar plates were similar. (D) Western blot analysis of OmpA in the membrane fraction of the wild-type RS218 and the nlpI mutant. The OmpA expression in the membrane fraction of the two strains was similar.