FIG. 5.

Comparative analysis of host cell cPLA₂α activation by the wild-type strain RS218 and its nlpI mutant. (A) Confluent monolayers of HBMECs were incubated with RS218 or its nlpI mutant for various time points. Cell lysates were then analyzed for phospho-cPLA₂α (p-cPLA₂α) or cPLA₂α by using specific antibodies in Western blot analysis. (B) p-cPLA₂α and cPLA₂α band intensities were estimated densitometrically and represented as the fold increase of p-cPLA₂α to cPLA₂α.