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(Top panel) Sensitivity of the real-time RT-PCR assay specific for Y. pestis rRNA. (Bottom panel) Correlation of quantitative results of bacterial culture and RT-PCR analyses for tissue samples positive with both assays. The results for samples that were determined to be positive by only one assay are described in the text. The line was derived from the regression equation relating numbers of genome equivalents and CFU for each tissue sample collected from moribund animals.
