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. 2010 May 10;78(7):2937–2945. doi: 10.1128/IAI.00144-10

FIG. 1.

FIG. 1.

P. aeruginosa mutants deficient for flagellum or flagellar stator proteins are resistant to phagocytosis by BMDCs in vitro. (A) C57BL/6 BMDCs were analyzed by FACS for relative association with GFP-transformed PA14, the pilus-deficient pilG mutant, or the flagellum-deficient flgK and fliN mutants. (B) C57BL/6 BMDCs were assayed by gentamicin protection assay for relative phagocytic uptake of PA14 or flagellum-deficient flgK and fliN mutants. The graph is plotted on a logarithmic scale. (C) FACS analysis (left) and gentamicin protection assay (right) of C57BL/6 BMDCs coincubated with either WT PA14 or the ΔmotAB ΔmotCD flagellar stator mutant. (D) FACS histogram of total BMDC cellular association with GFP-transformed P. aeruginosa strains after 45 min of coincubation. (E) Fluorescence microscopy of RFP-expressing BMDCs coincubated with GFP-transformed PA14 or the ΔmotAB ΔmotCD mutant viewed with DIC overlay at ×65 magnification. For all graphs, phagocytic uptake levels were normalized as percentages of the mean WT phagocytosis. For all genotypes, n is ≥9; means, standard deviations, and statistical significance (asterisks) are shown.