FIG. 6.

rCPB-induced disruption of the actin cytoskeleton in PAEC: fluorescent staining of F-actin in PAEC after 1 and 3 h and in porcine fibroblasts after 3 h of incubation with serum-free medium (SFM), 32 ng/ml rCPB, 32 ng/ml rCPB preincubated with MAb-CPB, and 3 μg/ml rCPB-Nus-Tag fusion protein. Disruption of the actin cytoskeleton was visible 1 h after exposure to rCPB. The effect increased over time (3 h) and was inhibited by neutralization of rCPB. The actin cytoskeleton of porcine fibroblasts was not affected.