FIG. 3.

Costimulatory molecule expression and IL-12 production are TLR4 dependent. Splenic DC were purified from naïve mice, plated, and incubated with anti-TLR4 (αTLR4) or isotype control antibody. Cells were stimulated overnight with irradiated E. cuniculi and stained for CD80, CD86, or MHC class II expression (A). Unstimulated cells are represented by the shaded histogram. The mean fluorescence intensity is presented next to each antibody treatment. IL-12 production by splenic DC was measured by intracellular staining after overnight treatment with monensin (B and C). Experiments were carried out twice, and the data are representative of one experiment.