TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristicsa	Source or reference
Strains
HB-1	Unencapsulated derivative of H44/76	8
HB-1 lbpAB::kan	Deletion of lbpA and lbpB	J. Kortekaas
HB-1 nalP::kan	Insertion of Kanr cassette in nalP	37
HB-1 app::kan	Insertion of Kanr cassette in app	37
HB-1 ausI::kan	Insertion of Kanr cassette in ausI	35
HB-1 iga::kan	Insertion of Kanr cassette in iga	37
H44/76		17
H44/76 nalP::kan	Insertion of Kanr cassette in nalP	37
Plasmids
pEN300	Camr; encodes wild-type NalP	37
pEN305	Camr; encodes active-site mutant NalP	37
pENLbpB(BNCV)	Camr; encodes LbpB from strain BNCV	R. Voulhoux

^aCam^r, chloramphenicol resistance; Kan^r, kanamycin resistance.