FIG. 7.

Immunoblot detection of PilV, PilX, and PilC1 in “classical” pilus preparations from a pilZ mutant. The WT strain and a pilE mutant were included as positive and negative controls, respectively. Equal amounts of pili were loaded in each lane, as assessed by PilE immunodetection (data not shown). As in Fig. 1, there was a major cross-reacting species (indicated by an asterisk) in the immunoblots in which minor pilins were detected, which might correspond to PilE.