FIG. 2.
Characterization of antibodies in the sandwich ELISA. (a) Botulinum neurotoxin type A complex assay using monoclonal antibody F1-2 as the capture antibody and F1-40 labeled with biotin as the detection antibody. (b) Botulinum neurotoxin type B complex assay using monoclonal antibody clone C3/4 IgG1 as the capture antibody and rabbit IgG-specific polyclonal antibody (Metabiologics) labeled with biotin as the detection antibody. (c) Botulinum neurotoxin type E complex assay using rabbit IgG-specific polyclonal antibody (Metabiologics) as the capture antibody and horse F(ab)2 fragments labeled with biotin as the detection antibody. (d) Botulinum neurotoxin type F complex assay using rabbit IgG-specific polyclonal antibody (Metabiologics) as the capture antibody and the same antibody labeled with biotin as the detection antibody. (e) E. coli O157 NCTC 13126 cells using Bactrace polyclonal antibody as the capture antibody and the same antibody labeled with horseradish peroxidase as the detection antibody. The symbols indicate the averages of three determinations, and the error bars indicate ±1 standard deviation.