FIG. 3.

AICC columns used for sensitive detection of botulinum neurotoxin complex A and E. coli O157. The limit of detection for the botulinum type A neurotoxin complex AICC assay was 0.5 ng. The limit of detection for the E. coli AICC assay was 500 CFU. Each column contained a negative-control frit toward the top of the column, a specific measurement filter in the middle, and a positive-control frit toward the bottom of the column. Measurement frits were separated with a spacer frit absorbed with 1% (wt/vol) BSA.