TABLE 1.
Specificity of the AICC assay for detection of botulinum neurotoxins in culture supernatants
| Strain | Detection witha: |
|||
|---|---|---|---|---|
| Botulinum neurotoxin type A AICC assay | Botulinum neurotoxin type B AICC assay | Botulinum neurotoxin type E AICC assay | Botulinum neurotoxin type F AICC assay | |
| Proteolytic C. botulinum strains | ||||
| Type A strain Eyemouth (subtype A1) | + | + | + | − |
| Type A strains NCTC 3805 (subtype A1), ATCC 3502 (subtype A1), NCTC 9837 (subtype A2), NCTC 2012 (subtype A3), CDC 657 (subtype Ba4), and H04464 107 [subtype A5(B)] | + | + | − | − |
| Type B strains NCIMB 10657 and NCTC 7273 | − | + | − | − |
| Type F strains ATCC 25764 and NCTC 10281 | − | − | − | + |
| Nonproteolytic C. botulinum strains | ||||
| Type B strain Eklund 17B | − | + | − | − |
| Type E strains Beluga, Sebald P34, and CDC 7854 | − | − | + | − |
| Type F strain Colworth 195 | − | − | − | − |
| Other strains | ||||
| C. botulinum type C strains Colworth 3334, Colworth 3335, and Colworth 3337 | − | − | − | − |
| C. botulinum type D strain NCIMB 10619 | − | − | − | − |
| C. sporogenes NCIMB 10696 | − | − | − | − |
| C. butyricum NCIMB 7423 | − | − | − | − |
a
+, detection; −, no detection.