TABLE 1.
Clone | Resistanceb | Gene function | Best-match organismc |
---|---|---|---|
AOAmox2 | Amo, Crb, Cft, Pip | Class A β-lactamase | Chitinophaga (54) (YP_003121133) |
AOCarb3 | Amo, Cft, Crb | Class A β-lactamase | Pedobacter (49) (ZP_01883167) |
AOCefta2 | Cft | Class A β-lactamase | USB (49) (ACH59002) |
AOAmox1 | Amo, Crb, Cft, Pip | Metallo-β-lactamase | Pseudomonas (46) (CAQ53840) |
AOCarb11 | Amo, Crb, Pip | Metallo-β-lactamase | USB (48) (ACH58985) |
AOCefta11 | Cft | Class C β-lactamase | Pseudomonas (74) (YP_349452) |
AOPip4 | Crb, Pip | Class C β-lactamase | Parachlamydia (29) (ZP_06299750) |
AOCarb2 | Crb | Class D β-lactamase | Chitinophaga (40) (YP_003120606) |
AOCefta1 | Amo, Crb, Cft | Class D β-lactamase | Legionella (81) (YP_123907) |
AOKan4 | Kan | AG acetyltransferase | USB (59) (AAS90627) |
AOKan6 | Kan | AG acetyltransferase | USB (59) (AAS90609) |
AOKan8 | Kan | AG acetyltransferase | USB (59) (AAS90609) |
AOTet43 | Tet | Efflux pump | Gordonia (71) (YP_003273675) |
The abbreviations are as follows: Amo, amoxicillin; Cft, ceftazidime; Crb, carbenicillin; Kan, kanamycin; Pip, piperacillin; Tet, tetracycline; USB, uncultured soil bacterium; AG, aminoglycoside.
Resistance refers to the antibiotics in the media on which E. coli cells bearing each fosmid clone were isolated.
BLAST analysis was performed as described previously (2). The values in parentheses are the percent amino acid identity of the best match followed by the GenBank accession number of the best match.