TABLE 3.
Secretory pathway and membrane-trafficking protein complexes identified in this study and protein subunit relative abundances
| Complexa | Reference | S. cerevisiae protein | A. niger locus tag | NSAF (10,000)b |
|
|---|---|---|---|---|---|
| Sorbitol | Xylose | ||||
| Sec62/Sec63 | 13 | Sec63 | An01g13070 | 16.9 | 14.8 |
| Sec66 | An16g08830 | 19.8 | 16.4 | ||
| Sec62 | An02g01510 | 14.3 | 9.1 | ||
| OT subcomplexes | 28 | Stt3 | An16g08570 | 11.4 | 15.7 |
| Ost3 | An02g14930 | 8.2 | 15.3 | ||
| Ost4c | An08g07485 (N terminus) | ND | ND | ||
| Ost1 | An02g14560 | 41.1 | 35.7 | ||
| Wbp1 | An07g04190 | 42.3 | 38.0 | ||
| Sec23-Sec24 heterodimer | 12 | Sec23 | An01g04730 | 9.0 | 4.1 |
| Sec24 | An08g10650 | 6.4 | 6.6 | ||
| p24 | 6 | Emp24 | An08g03590 | 12.2 | 17.3 |
| Erv25 | An01g08870 | 16.7 | 22.7 | ||
| Erv41/Erv46 | 39 | Erv41 | An03g04940 | 7.5 | 11.1 |
| Erv46 | An01g04320 | 12.5 | 14.9 | ||
| AP-1 adaptor | 62 | Apl2 | An16g02490 | 2.6 | 0.8 |
| Apm1 | An07g03200 | 2.8 | 1.5 | ||
| Apl4 | An01g02600 | 2.6 | 1.4 | ||
| Retromer complex inner shell | 49 | Pep8 | An01g07320 | 9.2 | 4.4 |
| Vps29 | An08g01030 | 10.0 | 5.1 | ||
| Vps35 | An16g04270 | 11.3 | 9.5 | ||
| Chaperonin-containing T complex | 27 | Tcp1 | An15g06370 | ND | ND |
| Cct2 | An01g06480 | 1.2 | 1.2 | ||
| Cct3 | An02g08920 | ND | ND | ||
| Cct4 | An02g12750 | 2.2 | 1.2 | ||
| Cct5 | An11g02360 | ND | ND | ||
| Cct6 | An16g03100 | ND | ND | ||
| Cct7 | An18g05770 | 0.2 | 1.0 | ||
| Cct8 | An04g07340 | ND | ND | ||
a
OT, oligosaccharyltransferase.
b
ND, not detected.
c
S. cerevisiae Ost4 is 36 amino acids long, too small for the 10-kDa cutoff used in this study.