FIG. 2.

(A) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of soluble and membrane proteins from cells grown on MMA alone and methanol plus ammonium. Bands highlighted (1 to 7) were cut from the gel and analyzed by matrix-assisted laser desorption ionization-mass spectrometry and tandem mass spectrometry. (B) Agarose gel photos of RT-PCR products showing the eight-gene cluster, which was induced by MMA. Agarose gel photos above each gene show the induction of each gene by MMA only, and gel photos above intergenic regions show that this gene cluster is cotranscribed. RT-PCR products using mRNA extracted from MMA-grown cells and methanol-grown cells are shown in lanes 1 and 3, respectively. Corresponding controls without reverse transcriptase are shown in lanes 2 and 4, respectively. Lane 5 is the positive control for PCR using genomic DNA from Methylocella silvestris.