TABLE 1.

List of oligonucleotides used in this study

Target	Sequence (5′-3′)		Product size (bp)	Annealing temp (°C)
	Forward primer	Reverse primer
Msil2632	GCGGCATAGTTGGATTGTTT	CATTCCAGCAAGATCGAACC	412	55
Msil2633	CTGCGAAAATCTTCGACCTC	GATCGAGCCTTTGACGAGAA	400	55
Msil2634	CTGAAAATTCCCGTCACCAT	TTCTCCCAATCGGTGATTTC	421	55
Msil2635	CAAAGAGTGTGGCGTCAAGA	TCTCGGTGATGACGTCGTAG	460	55
Msil2636	CGTGCTCAATCTCTGCCATA	GAATCCCTTGGTGGTTTCAA	327	55
Msil2638	GGCCGCAATATTTCCCTATT	TCGCAGATTGTGATCTCAGC	510	60
Msil2639	GACCGTGTTTGACGTCTCG	TCCGTCAACGCATGATAGAG	250	60
Msil2632/Msil2633	CCGATCAATATGTCGCCTTC	TGACGTCGCCTTTCACATAG	423	55
Msil2633/Msil2634	GCGCCTTGTATCATTTCCAC	GCGCTTGGAGATTTTCTGAC	598	55
Msil2634/Msil2635	CGCCTTGCAAACTACCTTTC	TATCAAGCCATGTCGCAAAG	411	55
Msil2635/Msil2636	CATCAGGAATGGCAGGATTT	CGAATTCCTTGGCGAGATAA	333	55
Msil2636/Msil2638	AATCGAAGGGCTGTTCTTCA	CGTGTCGCCGAAATATCC	591	60
Msil2638/Msil2639	CGCTGAACGATCGCAAAC	GAGCGACGCCAACAGCTC	547	60
Construction of gmas mutant	CCTGAGAATTCATGATCATGTCGGCATCC	CTGGAAGGATCCTCAGCAGTCGAGCGTCTGCTC	1,308	52
Amplification of kan gene cassette	GGTAGGTCGACGCATGCGAGCTCGGAAAGCCACGTTGTGTCTC	GGTAGGTCGACGCATGCGAGCTCAAGGTGTTGCTGACTCATAC	1,184	55
Confirmation of gmas::kan mutant	GCCTTGCAAACTACCTTTCG	CGAATTCCTTGGCGAGATAA	2,591 (for mutant), 1,665 (for wild type)	55