TABLE 4.
Gene description and BLAST search results
| ORF | Length (aa) | Top BLASTP match (identity [%]) | Gene assignmenta |
Note | |
|---|---|---|---|---|---|
| a | b | ||||
| Msil2632 | 299 | Glutamine amidotransferase, class II (Azorhizobium caulinodans ORS 571 [67]) | gltB1 | mgsA | Contains a GlxB-like glutamine amidotransferase domain |
| Glutamine amidotransferase, class II (Xanthobacter autotrophicus Py2 [65]) | |||||
| Msil2633 | 235 | Glutamate synthase domain 3-like protein (Methylobacterium populi BJ001 [72]) | gltB3 | mgsB | Contains a GXGXG motif commonly found in glutamate synthase |
| Glutamate synthase family protein (Xanthobacter autotrophicus Py2 [66]) | |||||
| Msil2634 | 444 | Putative glutamate synthase GltB2 subunit (Methylobacillus flagellatus KT [87]) | gltB2 | mgsC | Contains a highly conserved FMN-binding domain in GltS |
| Glutamate synthase domain 2-like protein (Azorhizobium caulinodans ORS 571 [86]) | |||||
| Msil2635 | 435 | Putative type III glutamine synthetase (Rhodopseudomonas palustris HaA2 [69]) | gltIII | gmas | Does not contain key residues for ammonium binding |
| γ-Glutamylmethylamide synthetase (Methylovorus mays [41]) | |||||
| Msil2636 | 416 | Putative sarcosine oxidase β subunit (Azorhizobium caulinodans ORS 571 [75]) | soxB | mgdA | |
| Sarcosine oxidase β subunit (Corynebacterium sp. P-1 [49]) | |||||
| Msil2637 | 95 | Putative sarcosine oxidase, δ subunit (Bradyrhizobium sp. BTAi1 [55]) | soxD | mgdB | |
| Sarcosine oxidase δ subunit (Corynebacterium sp. P-1 [37]) | |||||
| Msil2638 | 984 | Putative sarcosine oxidase α subunit (Methylobacterium populi BJ001 [55]) | soxA | mgdC | Contains two domains of the glycine cleavage T-protein |
| Sarcosine oxidase α subunit (Corynebacterium sp. P-1 [36]) | |||||
| Msil2639 | 209 | Putative sarcosine oxidase γ subunit (Rhodopseudomonas palustris HaA2 [37]) | soxG | mgdD | |
| Sarcosine oxidase γ subunit (Corynebacterium sp. P-1 [31]) | |||||
a
a, assigned based on BLASTP matches; b, assigned based on the similarity between Msil2632 to Msil2639 and the gene cluster of Methyloversatilis universalis (24).