FIG. 4.

braI_XEN, xenI2, and bxeR promoter activities in wild-type and QS mutant strains of B. xenovorans LB400^T. Bacterial cultures were started with an initial inoculum of 5 × 10⁶ CFU in 20 ml of KB-Tc medium, and the β-galactosidase activities were measured over 12 h of growth. All experiments were performed in triplicate, and means values with standard deviations are indicated in the graph. ANOVA in combination with Dunnett's post-test and were performed with Prism 4.0 software (GraphPad). A P value of <0.05 was considered significant (*).