TABLE 1.
B. kururiensis, B. xenovorans, B. unamae, P. putida, and E. coli strains and plasmids used in this study
| Strain or plasmida | Characteristicsb | Reference or source |
|---|---|---|
| Strains | ||
| B. kururiensis M130 | Ampr Rifr, isolated from surface-sterilized roots of rice | 5 |
| M130BRAI | braI::Km of B. kururiensis M130 | 73 |
| M130BRAR | braR::Km of B. kururiensis M130 | 73 |
| M130RSAL | rsaL::Km of B. kururiensis M130 | 73 |
| B. xenovorans LB400T | Type strain | 31 |
| LB400BRAI | braI::Km of B. xenovorans LB400T | This study |
| LB400BRAR | braI::Km of B. xenovorans LB400T | This study |
| LB400XENI2 | xenI2::Km of B. xenovorans LB400T | This study |
| LB400XENR2 | xenR2::Km of B. xenovorans LB400T | This study |
| LB400BXER | bxeR::Km of of B. xenovorans LB400T | This study |
| B. unamae MTl-641T | Maize rhizosphere isolate | 10 |
| UNABRAI | braI::Km of B. unamae MTl-641 | This study |
| UNABRAR | braR::Km of B unamae MTl-641 | This study |
| P. putida F117 | AHL-negative derivative of P. putida IsoF; PpuI− | 63 |
| E. coli M15 | NaI Str Rif Thi Lac− Ara+ Gal+ Mtl− F− RecA+ Uvr+ Lon+ | Qiagen |
| Plasmids | ||
| pRK2013 | Tra+ Mob+ ColE1 replicon; Kmr | 27 |
| pMOSBlue | Cloning vector; Ampr | Amersham-Pharmacia |
| pGEM-T Easy | Cloning vector; Ampr | Promega |
| pMP220 | Promoter probe vector, IncP, LacZ; Tetr | 70 |
| pLAFR3 | Broad-host-range cloning vector, IncP1; Tetr | 72 |
| pKNOCK-Km | Conjugative suicide vector; Kmr | 2 |
| pLZ1 | pLAFR3 containing B. unamae DNA | This study |
| pBBRMCS-5 | Broad-host-range vector Gmr | 42 |
| pKRC-12 | pBBR1MCS-5 carrying PlasB-gfp (ASV) Plac-lasR; Gmr | 63 |
| pMOS-LZ-1 | pMOSBlue carrying an 8-kb fragment containing partial B. kururiensis QS genes; Ampr | This study |
| pKNOCK-UNAI | Internal PCR braIUNA fragment of B. unamae cloned in pKNOCK-Km | This study |
| pKNOCK-UNAR | Internal PCR braRUNA fragment of B. unamae cloned in pKNOCK-Km | This study |
| pKNOCKXENR | Internal PCR braRXEN fragment of B. xenovorans cloned in pKNOCK-Km | This study |
| pKNOCKXENI | Internal PCR braIXEN fragment of B. xenovorans cloned in pKNOCK-Km | This study |
| pKNOCKXENI2 | Internal PCR xenI2 fragment of B. xenovorans cloned in pKNOCK-Km | This study |
| pKNOCKXENR2 | Internal PCR xenR2 fragment of B. xenovorans cloned in pKNOCK-Km | This study |
| pKNOCKBXE | Internal PCR bxeR fragment of B. xenovorans cloned in pKNOCK-Km | This study |
| PMPXENI1 | promoter of gene braIXEN cloned in pMP220 vector | This study |
| pMPX2I | Promoter of gene xenI2 cloned in pMP220 vector | This study |
| pMPBXE | Promoter of gene bxeR cloned in pMP220 vector | This study |
| pMP2786 | Promoter of gene Bxe_B2786 cloned in pMP220 vector | This study |
| pMP0016 | Promoter of gene Bxe_B0016 cloned in pMP220 vector | This study |
| pBBRXENI | braIXEN cloned into pBBR-MCS5 Gmr | This study |
| pMPUNAI | promoter of gene braIUNA cloned in pMP220 vector | This study |
| pGEMXENR1 | braRXEN cloned into pGEM; Ampr | This study |
| pGEMX2R | xenR2 cloned into pGEM; Ampr | This study |
| pQEXENR1 | braRXEN cloned into pQE30 expression vector; Ampr | This study |
| pQEXENR2 | xenR2 cloned into pQE30 expression vector; Ampr | This study |
| pQEBXER | bxeR cloned into pQE30 expression vector; Ampr | This study |
| pQEUNAR | braRUNA cloned into pQE30 expression vector; Ampr | This study |
| pQE30 | Expression vector, ColE1 replication origin, T5 promoter, His epitope; Ampr | Qiagen |
a
A superscript “T” indicates a type strain.
b
Cmr, chloramphenicol resistance; Gmr, gentamicin resistance; Tetr, tetracycline resistance; Ampr, ampicillin resistance; Rifr, rifampin resistance; Kmr, kanamycin resistance.