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Cell growth, glycerol utilization, and product synthesis in strains LA01 and LA02 containing mutations in the pathways that lead to the synthesis of competing by-products. LA01 is a double mutant (ΔpflB ΔfrdA) and LA02 a triple mutant (ΔadhE Δpta ΔfrdA). (A) d-Lactate production, cell growth, and glycerol consumption in shake flask cultures grown for 36 h. (B) Product yield for the cultures represented in panel A. Error bars represent standard deviations from triplicate measurements.
