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Chromatogram of 36-h fermentation samples taken from cultures of wild-type MG1655 (gray line) and LA02(pZSglpKglpD) (black line) demonstrating the chemical purity of lactate produced in the engineered strain. The inset shows the quantification of d- and l-lactate in the LA02(pZSglpKglpD) sample to assess chiral purity: the concentration (bars; g/liter) and percentage (number in parenthesis; wt/wt) of each enantiomer are shown.
