TABLE 1.
Primer sequences used for kinetic real-time PCRa
| SNP | Primer sequence (5′-3′) | ΔCT |
|---|---|---|
| fadD(234) | (F) GATTTCTCCWGTYTGCAYCTTTCY | 3 |
| (R) CCTTCCAGCAGATACTGTCCG | ||
| (R) CCTTCCAGCAGATACTGTCCT | ||
| (R) CCTTCCAGCAGATACTGTCCA | ||
| clpX(267) | (F) AGCGYGGKATTGTCTACATC | 3 |
| (R) ACCGGAAACGTCHCGDGTAACA | ||
| (R) ACCGGAAACGTCHCGDGTAACG | ||
| uidA(138) | (F) CCRGGAATGGTGATYACMGGC | 5 |
| (F) CCRGGAATGGTGATYACMGGT | ||
| (R) AGASRATYACGCTGCGATGG | ||
| clpX(177) | (F) AAACATYATTCAGAARCTGTTGCCA | 3 |
| (F) AAACATYATTCAGAARCTGTTGCCG | ||
| (R) GAGAAATYTTGTCRATYTMATCGAT | ||
| clpX(234) | (F) CTGTTGCARAARTGCGAYTAYG | 10 |
| (R) TTGTCYGACTTACGAGAAATYTTGTCG | ||
| (R) TTGTCYGACTTACGAGAAATYTTGTCA | ||
| lysP(198) | (F) GGTACAACTGGGCGGTGACYAGC | 15 |
| (F) GGTACAACTGGGCGGTGACYAGT | ||
| (R) CGGTGTATCCGGGAACCA | ||
| icdA(177) | (F) ATTCYCTTCCCRRAACATTGC | 3 |
| (F) ATTCYCTTCCCRRAACATTGT | ||
| (R) TTGCGTATTCRATCGCKGC | ||
| mdh(450) | (F) AACGYATCCAGAACGCG | 10 |
| (F) AACGYATCCAGAACGCA | ||
| (R) GGTTGCMGACCCRCCA |
a
SNPs are shown in bold. The difference in cycle threshold times between the matched and mismatched primer reactions (ΔCT) is depicted as numbers.