TABLE 2.
Growth parameters of the G. oxydans N44-1 strains in fermentors with 15% dissolved oxygen and 50 g liter−1 glucosea
G. oxydans strain | Final OD600 | CDW (g liter−1) | μb (h−1) | tdc (h) | Yx/s (g CDW g glucose−1)d |
---|---|---|---|---|---|
N44-1 pBBR1MCS4 | 9.0 ± 0.11 | 2.1 ± 0.05 | 0.18 ± 0.02 | 3.9 | 0.09 ± 0.005 |
N44-1 mgdH::kan pBBRMCS4 | 18.7 ± 0.19 | 4.4 ± 0.09 | 0.25 ± 0.05 | 2.8 | 0.13 ± 0.005 |
N44-1 ΔmgdH sgdH::kan pBBR1MCS4 | 24.4 ± 0.19 | 5.7 ± 0.07 | 0.32 ± 0.03 | 2.2 | 0.14 ± 0.002 |
Cultivation was carried out in bioreactors (Dasgip, Jülich, Germany) under pH and aeration control (Fig. 2).
μ, growth rate.
td, doubling time.
Final dry weight per liter divided by the substrate consumed: initial glucose (g liter−1) − residual glucose (g liter−1). Yx/s, growth yield coefficient.