TABLE 2.
One-step real-time RT-PCR primers and hydrolysis probes specific to European subtype TBEV and IPC mengovirus vMC0
| Target and description | Primer name | Sequence (5′ → 3′) | Position on genea | Product size (bp) |
|---|---|---|---|---|
| TBEV E gene | ||||
| Forward | TBEE-F6 | GGCTTGTGAGGCAAAAAAGAA | 1329-1349 | 87 |
| Reverse | TBEE-R2 | TCCCGTGTGTGGTTCGACTT | 1397-1416 | |
| Probe | TBEE-P4 | FAM-AAGCCACAGGACATGTGTACGACGCC-BHQ-1 | 1349-1374 | |
| Mengovirus vMC0 5′ noncoding region | ||||
| Forward | Mengo-F1 | GACTACCCACTCCCCCTTTC | 64-84 | 103 |
| Reverse | Mengo-R1 | GCTTCGGCCAGTAATGTGAT | 147-167 | |
| Probe | Mengo-P1 | JOE-TGAAGGCTACGATAGTGCCAGGGC-BHQ-1 | 88-112 |
a
For the TBEV E gene, positions of genes are according to accession number U27495.1. For the mengovirus vMC0 5′ noncoding region, positions are according to http://virology.wisc.edu/acp/Plasmids/PlasmidFiles/pMC0.gb.txt.