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Precision of the one-step duplex real-time RT-PCR
| Target and copies/μla | CV (%)b |
|
|---|---|---|
| Intra-assay precision | Interassay precision | |
| TBEV E gene | ||
| 100 | 25.40 | 35.05 |
| 1000 | 9.16 | 36.65 |
| 10,000 | 17.11 | 29.89 |
| Mengovirus vMC0 5′ noncoding region | ||
| 100 | 37.99 | 41.18 |
| 1000 | 24.83 | 41.47 |
| 10,000 | 26.35 | 37.61 |
Number of copies of TBEV or IPC mengovirus vMC0 standard RNA.
Calculated on the basis of assessed copy numbers.
