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. 2010 May 7;76(13):4185–4193. doi: 10.1128/AEM.00437-10

FIG. 4.

FIG. 4.

Southern blot analysis of gcvT in rhizobia. Genomic DNAs from B. liaoningense USDA3622 (lane 1), R. galegae USDA4128 (lane 2), R. hainanense USDA3588 (lane 3), R. huautlaense USDA4900 (lane 4), R. tropici USDA9030 (lane 5), M. amorphae USDA1001 (lane 6), S. fredii USDA205 (lane 7), S. meliloti USDA1002 (lane 8), S. terangae USDA4894 (lane 9), R. leguminosarum USDA2370 (lane 10), and S. saheli USDA4893 (lane 11) were restriction enzyme digested with EcoRI and separated electrophoretically in 0.8% agarose. The gel was blotted onto nitrocellulose and probed with the 32P-labeled S. fredii USDA257 gcvT gene. Molecular weight markers in kilobases are shown on the left side of the figure.