TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| S. fredii USDA257 | Nod+ on soybean cultivar Beijing | USDA-ARS |
| S. fredii USDA205 | Nod+ on soybean cultivar Beijing | USDA-ARS |
| S. meliloti USDA1002 | Nod+ on alfalfa | USDA-ARS |
| S. terangae USDA4894 | Nod+ on acacia | USDA-ARS |
| B. liaoningense USDA3622 | Nod+ on soybean | USDA-ARS |
| R. galegae USDA4128 | Nod+ on Galega spp. | USDA-ARS |
| R. hainanense USDA3588 | Nod+ on Desmodiumspp. | USDA-ARS |
| R. huautlaense USDA4900 | Nod+ on Sesbania herbacea | USDA-ARS |
| R. tropici USDA9030 | Nod+ on Phaseolus vulgaris | USDA-ARS |
| R. leguminosarum USDA2370 | Nod+ on pea | USDA-ARS |
| M. amorphae USDA1001 | Nod+ on Amorpha fruticosa | USDA-ARS |
| S. fredii USDA191 | Nod+ on soybean | USDA-ARS |
| S. fredii HBK257-15A | USDA257 derivative containing a Tn5 insertion in gcvT; Kmr | This work |
| S. fredii HBKgcvT-Ω | USDA257 derivative containing an Ω insertion in gcvT; Specr | This work |
| S. fredii HBKgcvP-Ω | USDA257 derivative containing an Ω insertion in gcvP; Specr | This work |
| E. coli HB101 | Restriction-minus, recA background | Takara Bio Inc. |
| E. coli DH5α | λ− φ80dlacZDM15 D(lacZYA-argF)U169 recA1 endA hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Gibco BRL |
| Plasmids | ||
| pGEM-7zf(+) | Apr | Promega |
| pBluescript II SK(+) | Apr | Stratagene |
| pGEM-T Easy | Apr | Promega |
| pMP220 | Tcr | 45 |
| pJQ200sk | Gmr | 38 |
| pGCV-1 | Kmr Tcr; USDA257 gcv-Tn5 in pLAFR1 | This work |
| pGCV-2 | Tcr; USDA257 gcv in pLAFR1 | This work |
| pGCV-3 | Apr; 1.5-kb USDA257 gcvTEcoRI fragment in pGEM-7zf(+) | This work |
| pGCV-4 | Apr; 5.7-kb EcoRII fragment containing part of gcvT and gcvHP in pBluescript II SK(+) | This work |
| pGCV-5 | Tcr; 1.5-kb EcoRI fragment from pGVC-3 cloned in pMP220 | This work |
| pGCV-6 | Apr; 3.4-kb PstI fragment from pHKCS-4 in pBluescript II SK(+) | This work |
| pGCV-6Ω | Apr Spcr; 2.0-kb Ω fragment cloned into EcoRI site of pHKCS-6 | This work |
| pGCV-7Ω | Gmr Specr; 5.4-kb PstI fragment from pHKCS-6Ω cloned in pJQ200sk | This work |