TABLE 2.
Epidemiological characteristics of IRT-producing isolates
| IRT | pI | Isolate | Phylogroup | ST (CC) | Origin | Source | Antibiotic resistancea | blaIRT location | Plasmid size (kb)b |
Replicon type | Conjugation transfer | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | TC | |||||||||||
| TEM-30 (IRT-2) | 5.2 | 10924 | B2 | ST131 | Hospital | Respiratory tract | Nal, Cip | Plasmid | 60, 7, 5 | 50, 7, 5 | IncFII | + |
| 44965 | B2 | ST12 (CC12) | Community | Urine | Chromosome | 50 | − | |||||
| 29904 | D | ST38 (CC38) | Hospital | Respiratory tract | SxT | Plasmid | 100, 45 | IncFc | − | |||
| 46836 | D | ST393 (CC31) | Community | Urine | Plasmid | NOd | 40 | IncN | + | |||
| TEM-32 (IRT-3) | 5.4 | 34351 | B2 | ST1045 | Hospital | Blood | SxT | Plasmid | 145, 48 | 145, 48 | IncFII | + |
| 161490 | A | ST46 (CC46) | Community | Urine | Chromosome | 97 | − | |||||
| TEM-33 (IRT-5) | 5.4 | 45995 | B2 | ST95 (CC95) | Hospital | Blood | Nal, Cip | Plasmid | 97, 30 | 97 | IncFII | + |
| 20561 | B2 | ST131 | Hospital | Urine | Nal, Cip, SxT | Plasmid | 70 | 60 | IncFII | + | ||
| 127618 | D | ST12 (CC12) | Community | Urine | Nal, Cip | NO | 100 | − | ||||
| TEM-34 (IRT-6) | 5.4 | 132028 | D | ST1216 (CC38) | Hospital | Urine | Nal, Cip | Plasmid | 145, 30 | IncFIB/FIIA | − | |
| TEM-36 (IRT-7) | 5.2 | 41392 | A | ST617 (CC10) | Community | Urine | Nal, Cip | Plasmid | 97, 48 | 97,48 | IncFII | + |
| TEM-37 (IRT-8) | 5.2 | 43340 | B2 | ST131 | Community | Urine | Plasmid | 97, 40 | IncFII | − | ||
| 18204 | D | ST117 | Community | Urine | Nal | Chromosome | 97 | − | ||||
| TEM-40 (IRT-11) | 5.4 | 162266 | A | ST393 (CC31) | Hospital | Urine | Nal, Cip, SxT | NO | NO | IncFc | − | |
| 47499 | B2 | ST1217 | Community | Urine | SxT | Chromosome | 97, 40 | − | ||||
| TEM-54 | 5.4 | 138942 | B2 | ST1218 | Hospital | Urine | SxT | Plasmid | 50, 30, 20 | 50 | IncN | + |
| 115819 | B2 | ST95 (CC95) | Community | Urine | Nal, Cip, SxT | Plasmid | 145, 60 | 145, 50 | IncFII | + | ||
| 133972 | B2 | ST88 (CC23) | Community | Urine | Plasmid | NO | 50, 15 | + | ||||
a
Nal, nalidixic acid; Cip, ciprofloxacin; SxT; sulfamethoxazole-trimethoprim.
b
Plasmid size was determined by hybridization of S1-digested DNA. Underlining corresponds to plasmids with blaIRT-positive hybridization. WT, wild type; TC, transconjugant.
c
Double sequence.
d
NO, not obtained due to continuous DNA degradation.