TABLE 1.

Primers used for amplification and sequencing of the 16S rRNA and groEL genes of “Candidatus N. mikurensis” in patient 1

Target	Primers used for:
	First PCR	Nested PCR	Sequencing
16S rRNA gene	8f + 1512r	8f + Nehr16S re	8f, Nehr16S re, Nehr16S seq1, Nehr16S seq2
		Nehr16S fo + 1512r	Nehr16S fo, 1512r, Nehr16S seq3, Nehr16S seq4
groEL	Nehrgro fo + Nehrgro re	Nehrgro fo + Nehrgro seq1	Nehrgro fo, Nehrgro seq1
		Nehrgro seq2 + Nehrgro seq4	Nehrgro seq2, Nehrgro seq4
		Nehrgro seq3 + Nehrgro re	Nehrgro seq3, Nehrgro re