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Primers used in this study
| Primer target (description)a | Primer sequences (forward/reverse) | Amplicon size (bp) | Tm (°C) |
|---|---|---|---|
| MTC ITS (single copy) | GCGAGAGCCGGGTGCATG | 47 | 75.8 ± 0.8 |
| AACAGTGTGTTGGTGGCCAA | |||
| IS6110 (multicopy) | GAACGGCTGATGACCAAACT | 72 | 83.1 ± 1.0 |
| ATCAGCGATCGTGGTCCTG | |||
| Beta-actin (control) | AGCGGGAAATCGTGCGTG | 135 | 83.0 ± 0.4 |
| GGTGATGACCTGGCCGTC |
MTC, Mycobacterium tuberculosis complex.
