TABLE 1.

Results of the NucliSENS EasyQ HPV test for NA extracts

NA extract	Fixationa	Cell type	NA input (ng/5μl)	NucliSENS EasyQ resultb
				U1A	HPV18	HPV16
Total RNA	Unfixed	HeLa	410	Pos	Pos	Neg
	PSC	HeLa	3033	Pos	Pos	Neg
	SP	HeLa	163	Neg	Pos	Neg
Total DNA	Unfixed	HeLa	81	Pos	Pos	Neg
	PSC	HeLa	123	Pos	Pos	Neg
	SP	HeLa	35	Neg	Pos	Neg
DNA-free RNA	Unfixed	HeLa	451	Neg	Pos	Neg
	Unfixed	HeLa	359	Pos	Pos	Neg
	Unfixed	HeLa	949	Pos	Pos	Neg
	Unfixed	CaSki	478	Neg	Neg	Neg
	Unfixed	HaCaT	465	Pos	Neg	Neg
	PSC	HeLa	2572	Pos	Pos	Neg
	PSC	HeLa	2493	Pos	Pos	Neg
	PSC	HeLa	990	Neg	Pos	Neg
	PSC	CaSki	1241	Neg	Neg	Pos
	PSC	HaCaT	493	Neg	Neg	Neg
	SP	HeLa	236	Neg	Pos	Neg
	SP	HeLa	58	Neg	Pos	Neg
	SP	HeLa	117	Neg	Pos	Neg
	SP	CaSki	87	Neg	Neg	Neg
	SP	HaCaT	79	Neg	Neg	Neg
RNA-free DNA	Unfixed	HeLa	30	Neg	Pos	Neg
	Unfixed	HeLa	18	Neg	Pos	Neg
	Unfixed	HeLa	47	Neg	Pos	Neg
	Unfixed	CaSki	10	Neg	Neg	Pos
	Unfixed	HaCaT	27	Neg	Neg	Neg
	PSC	HeLa	11	Neg	Pos	Neg
	PSC	HeLa	36	Neg	Pos	Neg
	PSC	HeLa	10	Neg	Pos	Neg
	PSC	CaSki	10	Neg	Neg	Pos
	PSC	HaCaT	14	Neg	Neg	Neg
	SP	HeLa	11	Neg	Pos	Neg
	SP	HeLa	15	Neg	Pos	Neg
	SP	HeLa	20	Neg	Pos	Neg
	SP	CaSki	13	Neg	Neg	Pos
	SP	HaCaT	17	Neg	Neg	Neg

^aPSC, PreServCyt; SP, SurePath.

^bPos, positive; Neg, negative.