TABLE 2.

Validation of reference real-time respiratory virus PCR assays^a

Multiplex assay	Virus target(s)	No. of specimens with pos PCR VL result/no. tested (% PCR pos)
		FA staining pos	FA staining neg, culture pos	FA staining neg, culture neg
1b	Influenza A virus	30/30 (100)	30/30 (100)	1/152 (0.7)
1b	Influenza B virus	31/31 (100)	27/30 (90)	0/152
3	Respiratory syncytial virus	30/30 (100)	9/9 (100)	6/39 (15)
4	Parainfluenza virus	16/16 (100)	8/11 (73)	0/10
5	Adenovirus	3/3 (100)	26/26 (100)	0/33
6	Human metapneumovirus	16/16 (100)	NAc	7/104 (7)c
6	Rhinovirus	NAd	27/27 (100)d	26/66 (39)d

^aNA, not applicable; pos, positive; neg, negative.

^bThe influenza virus multiplex assay detects both influenza A and B viruses and can distinguish between them with different probe labels.

^cNo cultures were performed for human metapneumovirus. Each of the seven specimens that were positive by reference PCR was also positive by other PCR assays (1).

^dNo FA staining was performed.