TABLE 2.
Primers used in this study
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size (bp) | Reference or source |
|---|---|---|---|---|
| flaB fragment 1 | TCATAAATCATAATACGTCAG | AATGTCCATGAAGCTTGTGA | 514 | This study |
| flaB fragment 2 | CTGAAGAGCTTGGAATGCAAC | AGGTACTTGATTTGCTTGTGC | 538 | This study |
| rrs fragment 1 | GTTTGATCCTGGCTTAGAAC | TTACAATCTTTCGACCTTCTT | 417 | This study |
| rrs fragment 2 | CACACTGGAACTGAGATACGG | TTCGCCTCTGGTATTCTTCCT | 419 | This study |
| rrs fragment 3 | TGCGTAAAATACCACAGCTCA | TGAGTCCCCATCTTTACATGC | 547 | This study |
| rrs fragment 4 | TACCAGGGCTTGACATATACA | GAGGTGATCCAGCCACACTTT | 561 | This study |
| rrf-rrl outer | GTATGTTTAGTGAGGGGGGTG | GGATCATAGCTCAGGTGGTTAG | 807a | 3 |
| rrf-rrl inner | AGGGGGGTGAAGTCGTAACAAG | GTCTGATAAACCTGAGGTCGGA | 765a | 3 |
| recC fragment 1 | AAGATATATAAAACAAACAAA | GTCAATTTCTCTAGTCTCTCC | 623 | This study |
| recC fragment 2 | ATTGAAACAAAGAGAATAATA | ATTTATTCATTACTTTTGTGT | 634 | This study |
| recC fragment 3 | GGAACGATTAGTAATTTTAA | TTTAAGGTATTGTATTTTTGT | 474 | This study |
| recC fragment 4 | ATATGAATGGGCAGAAATAAT | TTATTGATATCTGGATTTAGA | 495 | This study |
| recC fragment 5 | GCAAGCTCTGATAAAATTGAA | TTGATGTTTATGGGAATTATT | 737 | This study |
| groEL fragment 1 | TGGCTAAGGACATATATTTTA | ATCTTTGCCAACTCTGTCCAT | 512 | This study |
| groEL fragment 2 | TTCTGCAAATAATGATACTTC | AACATTCTCAAGAGTAAGTCC | 487 | This study |
| groEL fragment 3 | ATTGCTATACTTACTGGAGG | TAAATAGAACTCTCAAATCCA | 524 | This study |
| groEL fragment 4 | AAGGTTTTGAGATTGTGAAGA | TTACATCATTCCCATTCCAG | 323 | This study |
| p66 fragment 1 | TTTAGATTTGATATGGATGA | GATATGTGTCCAAGTATAGA | 899 | This study |
| p66 fragment 2 | TTCCTCAATAACATATGGTCT | ACACTTCCATTTTGATCTTT | 945 | This study |
| glpQ fragment 1 | CATTAATTATAGCTCACAGAG | AACAAGCATTATCAATTTTCC | 599 | This study |
| glpQ fragment 2 | TATGGCATAAACAACAAGGTA | AATCTGTAAATAGACCATCTA | 453 | This study |
a
The amplicon size may vary among species.