TABLE 1.

Rates of Hsp104 curing of [PSI⁺]^a

Strain	% [psi−] cells/generation
Wild type plus empty vector	<0.001
Wild type plus Hsp104	9.0 ± 0.3
Wild type plus Hsp104ΔC4	8.9 ± 0.6
sti1Δ mutant plus Hsp104	0.2 ± 0.2
hsc82Δ mutant plus Hsp104	0.3 ± 0.2
hsp82Δ mutant plus Hsp104	4.1 ± 0.2

^aHsp104 (or Hsp104ΔC4 where indicated) proteins were overexpressed from copper-inducible alleles on pMR26 and pMR81, respectively. Cells removed periodically from cultures grown for four to six generations were spread onto 1/2YPD to determine the proportion of [psi⁻] (entirely red) colonies. At each time point, 500 to 1,500 colonies were assessed. Values are averages of the results for at least three experiments ± standard deviation (n = 3 to 19). Generations were measured as doublings of culture OD₆₀₀. No [psi⁻] colonies were detected in cultures that did not overexpress Hsp104 (first row).