FIG. 2.
Mutations in histone H2B core residues render yeast cells sensitive to DNA-damaging agents. (A) Growth phenotypes of histone H2B alanine mutant strains in the presence of the DNA-damaging agent MMS. Tenfold serial dilutions of each strain (starting with approximately 5 × 105 cells) were spotted on SC-Trp medium or SC-Trp medium containing the indicated concentrations of MMS. (B) Histone H2B core mutant strains were examined for sensitivity to UV irradiation at doses of 0, 50, and 100 J/m2. The percentage of viable cells in each strain was plotted as a function of UV dose. Only the histone H2B alanine mutant strains with a significant UV sensitivity phenotype are depicted. WT, wild type.