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Pbx1 protein-protein interactions in osteoblasts. (A) Whole-cell lysates from confluent MC3T3-E1 cells were immunoprecipitated with Hoxa10, Runx2, Pbx1, HDAC1, or control (IgG) antibodies. Proteins were resolved by SDS-PAGE, and Hoxa10 and Pbx1 were detected by Western blotting with specific antibodies. (B) Lysates from MC3T3-E1 were used for immunoprecipitation using anti-HDAC1 followed by Western blotting with anti-Pbx1 or specific HDAC antibody.
