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. 2010 May 17;30(14):3661–3671. doi: 10.1128/MCB.00201-10

FIG. 2.

FIG. 2.

Ectopic expression of Figla in male germ cells. (A) Transgene containing the Figla genomic locus (4.3 kbp) downstream of the human TSPY1 promoter (2.4 kbp), which is expressed in spermatogonia and early spermatocytes. Exons are represented by Arabic numbered red boxes. (B) Figla transcripts in normal and TSPY1-Figla transgenic ovaries and testes at D1 and D18 were detected by qRT-PCR and are expressed as means ± SEM of total RNA isolated from three biological samples. Gapdh was used as an endogenous template control. (C) The same experiment as shown in panel B, but using testicular RNA from D1 to 10-week-old TSPY1-Figla transgenic mice. (D) Transcript abundance levels of seven testis genes (means ± SEM) were determined by qRT-PCR relative to Gapdh in total RNA isolated from different organs harvested from 2-month-old TSPY1-Figla transgenic mice. Lvr, liver; Kid, kidney; Br, brain; Hrt, heart; Lg, lung; Ov, ovary. (E) The same experiment as shown in panel D, but using total RNA isolated from different organs in normal mice. (F) In situ hybridization of normal testes using antisense probes specific for 4930511l11Rik, 1700009N14Rik, 1700019M22Rik, 1700011E24Rik, Irgc1, and Slc2a3. Arrows highlight localization of transcripts.