FIG. 3.
The addition of an MTE increases the affinity of TFIID for the core promoter. The wild-type (WT) and WT+MTE (containing the Tollo MTE sequence from +18 to +27, relative to the A+1 position in the Inr) versions of the Drosophila E74B (A) and Doc (B) core promoters were subjected to DNase I footprinting analysis with purified Drosophila TFIID. The positions of the Inr (−2 to +4), MTE (Tollo core promoter sequence from +18 to +27), and DPE (+28 to +33) motifs are indicated. In the WT+MTE promoters, regions of DNase I protection and hypersensitivity are indicated by brackets and filled dots, respectively.