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. 2010 May 10;30(14):3471–3479. doi: 10.1128/MCB.00053-10

FIG. 4.

FIG. 4.

The TAF6 and TAF9 subunits of TFIID appear to be in close proximity to the MTE. (A) Diagram of photoaffinity probes containing AB-dUMP at the +20, +25, or +30 position relative to the +1 transcription start site. The Tollo core promoter sequences are shown. The diagram is roughly to scale. (B) Photo-cross-linking of purified TFIID with the Tollo core promoter. Reactions were performed in the presence or absence of TFIID as well as with or without UV irradiation, as indicated. (C) Photo-cross-linking of purified TFIID with the E74B core promoter containing the Tollo MTE. The E74B+MTE core promoter is identical to the construct that was used in the DNase I footprinting analysis shown in Fig. 3A. Reactions were performed as described for panel B.