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The effects of silencing of Rap1GAP on AJ proteins are Rap dependent. Rap1GAP-depleted (E11) and control (Scr) cells were transiently transfected with Rap1- and Rap2-directed (+) versus scrambled (−) siRNAs. After 48 h, cells were fixed and stained for E-cadherin (A) or p120-catenin (B). Note the accumulation of p120-catenin at cell/cell contacts in Rap1/2-depleted cells (indicated by arrows). Total cell lysates prepared from replicate plates were analyzed for Rap expression.
