FIG. 2.
Characterization of the Cot-1− domain. A single representative blastomere is shown in all panels, and the intensity of immunostaining is quantified by densitometry across the indicated path (′ to ″) and plotted in the relative intensity range of 0 to 1. (A to C) Immunostaining using antibodies against RNA polymerases II and III as indicated. The frequencies of exclusion from XP were the following: Pol-II (CTD), 59% (n = 17); Pol-II (H5), 79% (n = 19); Pol-III, 58% (n = 19). (D to F) Expression analysis of the indicated repetitive elements by RNA FISH. Exclusion frequencies were the following: LINE, 62% (n = 13); B1, 67% (n = 12); and B2, 64% (n = 14).