FIG. 5.

Genic silencing depends on Xist. (A to D) Biallelic expression of indicated genes in Xist-deficient preimplantation embryos of different stages. Shown are merged z sections taken through the nucleus to capture signals in all focal planes. (E to H) Summary of genic expression in Xist mutant female (F) and male (M) embryos of the indicated stage. n, number of nuclei examined; the number of embryos analyzed is in parentheses. (I) Allele-specific RT-PCR of seven X-linked genes in wild-type (WT) and Xist mutant morulae produced by the indicated crosses. Mus, M. musculus; Cas, M. castaneus; M, maternal; P, paternal; PBS, negative control derived from the wash fluid after embryos are isolated to rule out contamination.